• 産品品牌： REAGEN
• 産品貨号： RNV81029
• 産品規格： 96T
• 産品适用： Scan檢測
• 産地來源： 中國深圳

1. Introduction

REAGEN Scan Kit is a Mycoplasma detection kit based on TaqMan qPCR technology. It can detect more than 200 Mycoplasma, Ureaplasma, Mesoplasma, Spiroplasma, Acholeplasma and Phytoplasma, and does not cross-react with eukaryotic cells. The 2 x Premix master mix provided in the kit contains mycoplasma-specific FAM-MGB probe, internal control (Internal Control, IC) DNA and its specific ROX probe.

2. Features

1. Wide coverage: detection coverage of up to 245 species of Mycoplasma contamination.

2. High sensitivity: the lowest detection limit can reach ≤10 CFU/ml.

3. Simple operation: it can be detected with 2 µL of cell culture medium, without the nucleic acid extraction process.

4. Save time: complete the test within 1.5 hours.

5. Good reproducibility: The optimized dual probe method and hot-start direct amplification Taq enzyme ensure the stability of the detection.

3. reagent

1. 2xPremix: 1 mL for 100 reactions

2. HS-Taq: 20 µL for 100 reactions

3. Positive control: 10 µL

* After receiving the goods, store at -20︒C; mix the enzyme with 2x premix before use, and store in aliquots and protected from light.

4. operation steps

A. Sample preparation

Collect the cell culture medium that has been cultured for at least 2 days.

B. PCR reaction

1. Add 10 µL of 2xPremix premixed with HS-Taq enzyme to each qPCR reaction tube.

2. Add 2 µL of the cell culture medium or control sample to be tested, and add 8 µL of sterile water to a total volume of 20 µL.

3. Start the program on the computer and select dual-channel FAM-MBG and ROX-None.

4. Amplification reaction conditions: 95 °C pre-denaturation for 5 min; 95 °C 15 sec, 60 °C 30 sec, 45 cycles.

C. Data analysis

1. Save the data;

2. Read the fluorescence intensity of FAM and ROX channels, FAM is the fluorescence signal of mycoplasma, and ROX is the internal control fluorescence signal;

3. Judge the test results based on the amplification curve and Ct value.